Figure 5
From: Orexin system is expressed in avian liver and regulates hepatic lipogenesis via ERK1/2 activation
Orexin regulates lipogenic program in LMH cell line. Orexin treatments affects lipogenic factors (ACC, FASN, ACLY, ME, SCD-1) and their transcription factors. Protein expression (a–c) was determined by Western blot and gene expression (e–h) was measured by qPCR. Phosphorylated levels of ACC was also assessed by immunofluorescence staining (d). Data are presented as mean ± SEM (n = 6/group). *Indicates significant difference from controls at P < 0.05. ACC acetyl-CoA carboxylase, ACLY ATP citrate lyase, FASN fatty acid synthase, INSIG-2 insulin-induced gene 2, ME malic enzyme, PPAR peroxisome proliferator-activated receptor, SCD-1 stearoyl-CoA desaturase-1, SREBP-1/2 sterol regulatory element-binding protein 1/2. Full-length blots/gels are presented in Supplementary Fig. S1.
