Figure 6 | Scientific Reports

Figure 6

From: Endoplasmic reticulum chaperone BiP/GRP78 knockdown leads to autophagy and cell death of arginine vasopressin neurons in mice

Figure 6

Pharmacological inhibition of autophagy exacerbates AVP neuronal loss in AVP neuron-specific BiP knockdown mice. (A) Representative images of immunofluorescence staining for AVP in the SON and PVN of chloroquine-treated mice 2 (cont sh 2wk + CQ) and 4 weeks after injection of rAAV-AVPp-scrambled shRNA (cont sh 4wk + CQ), and 2 (BiP sh 2wk + CQ) and 4 weeks after rAAV-AVPp-BiP shRNA injection (BiP sh 4wk + CQ). Scale bars: 50 μm (SON), 100 μm (PVN). The number of AVP neurons in the SON (B) and PVN (C) in the cont sh 2wk + CQ, cont sh 4wk + CQ, BiP sh 2wk + CQ and BiP sh 4wk + CQ groups. Results were analyzed by two-way ANOVA followed by a Bonferroni test and are expressed as the means ± SE (n = 3 per group). n.s. not significant.

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