Figure 1 | Scientific Reports

Figure 1

From: Histo-ELISA technique for quantification and localization of tissue components

Figure 1

Experimental flowchart. Seven macro-sections of 1 mm in thickness were cut with brain slicer starting from the brain pole. The second (a), fourth (b) and sixth (c) were collected and embedded for farther cryo-serial sections. Three 10 µm thick cryo-serial sections were prepared for each macro-section and supposed to be for (1) ABC Immuno-staining colorized with DAB for the purpose of localization of interest proteins, (2) Histo-ELISA/HE colorized with TMB and the sequential HE staining, and (3) Histo-ELISA blank control without antibody application.

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