Table 1 HPRT activity.

From: Metabolic interaction between amino acid deprivation and cisplatin synergistically reduces phosphoribosyl-pyrophosphate and augments cisplatin cytotoxicity

Condition

Enzyme activity (nmol/min/mg protein)

Control

1.10 ± 0.19

Lys−

1.21 ± 0.17

CDDP

1.14 ± 0.16

Lys−/CDDP

1.03 ± 0.18

  1. Dih10 mouse hepatoma cells were incubated for 6 h in full medium (Control), lysine-free medium (Lys−), full medium containing 100 µM cisplatin (CDDP), or lysine-free medium containing 100 µM cisplatin (Lys−/CDDP). The cells were extracted, and HPRT activity in the extracts was measured following [8-14C] hypoxanthine incorporation into nucleotides in the presence of 1 mM PRPP. Data are the mean ± S.D. of three independent experiments.
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