Table 2 Mouse serum amino acid concentrations.

Amino acid	Control	Lys⁻	CDDP	Lys⁻/CDDP
	µmol/L
Ala	382	355	382	522
Arg	115	108	117	117
Asn	36	33	32	39
Asp	22	21	19	17
Cys	5.5	3.9	4.8	4.0
Gln	505	464	442	447
Glu	72	56	61	51
Gly	391	415	393	636
His	81	95	91	92
Ile	80	92	98	86
Leu	129	152	147	130
Lys	296	141***	304	129***
Met	57	54	83	96
Phe	64	71	74	70
Pro	40	44	41	51
Ser	162	192	173	220
Thr	216	209	217	278
Trp	40	34	34	34
Tyr	68	47	64	56
Val	153	185	189	192

Mice were injected in the flank with Dih10 hepatoma cells, and received either normal rodent chow (Control) or three cycles of lysine-deficient chow (Lys^-) for 5 days followed by 3 days of normal chow as depicted in Fig. 4A. Some animals on either normal chow or lysine-deficient chow received one injection of cisplatin (CDDP). At the end of the third cycle of the lysine-deficient diet, the animals were euthanized, and blood was obtained by cardiac puncture. Amino acids in the serum were measured by high performance liquid chromatography coupled to post-column derivitization with ninhydrin. Data are mean values from five animals in each group; *** = p < 0.001 compared to control animals.

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