Figure 6
From: The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata
Hemagglutination of red blood cells by SeviL. 20Â \(\upmu\)L of a two-fold dilution of SeviL (initially 10Â mg/mL) were serially diluted and added to the same volume of a red cell suspension containing 0.25% Triton-X100 and 10Â mM calcium chloride, and incubated for 1Â h at room temperature. Appearance of a dot indicates no agglutination. The number over the wells indicates the protein dilution factor. Top row: dimer SeviL, middle row: dimer SeviL (D39H), and bottom row: monomer SeviL (Q12R/F126K). Column C is a negative control with no added lectin.
