Figure 2

Fluorescence correlates with proliferation in MITO-Luc/GFP zebrafish line. (A) Flow cytometry analysis of cells dissociated from 200 zebrafish MITO-Luc/GFP 40 hpf embryos, previously incubated for 3 h with BrdU. Fixed cells were stained with anti-BrdU and anti-GFP antibodies. Histogram plot represents different BrdU accumulation in GFP positive (red) and negative (blue) cells. (B) GFP positive and GFP negative cells gating strategy. (C) GFP signal in 6 (I), 24 (II) and 33 hpf (III) embryos. GFP expression is almost ubiquitous throughout 6 and 24 hpf embryos (I,II). It is tissue specific in 33 hpf embryos being accumulated in the head (III, red arrow), proximal trunk (III, white arrows) and in developing fin (III, yellow arrow). Bright light images are shown (IV,V,VI).