Figure 4
From: MITO-Luc/GFP zebrafish model to assess spatial and temporal evolution of cell proliferation in vivo
Proliferation markers colocalize with GFP and luciferase proteins in MITO-Luc/GFP zebrafish tissues. (A) Immunofluorescence analysis of DAPI (I), pH3 (II), GFP (III), HuC/D(IV), DAPI-pH3 merge (V), pH3-HuC/D merge (VI), pH3-GFP merge (VII) and GFP-HuC/D merge (VIII) in the trunk region of 24 hpf whole-mount transgenic embryos. (B) Immunofluorescence analysis of luciferase (I), GFP (II, IV), pH3 (III), luciferase-GFP merge (V) and pH3-GFP merge (IV) in adult epidermal tissue. (C) Immunofluorescence analysis of GFP (I,IV), PCNA (II,V), GFP-PCNA merge (III,VI), in intestine (I,II,III) and ovary (IV,V,VI). (D) Immunohistochemical analysis of luciferase (I, III, V, VII), PCNA (II, IV), pH3 (VI, VIII) and GFP (IX) in adult intestine (I, II), testes (III, IV), kidney (V, VI) and gills (VII, VIII, IX). In all tissue analyzed, cells expressing proliferation markers, either PCNA or pH3, show immunoreactivity for luciferase and/or GFP.
