Figure 7
Evaluation of renal oxidative stress and hypoxia at 14 and 28 days postischemia and CD34 protein expression at 14 days postischemia. (a) Protein expression of malondialdehyde (MDA) at 14 and 28 days postischemia in the IR kidneys. (b) Western blot analysis of renal HIF-1α and α-tubulin in the IR kidneys at 14 and 28 days postischemia. (c) Western blot analysis of CD34 and α-tubulin in the IR kidneys at 14 days postischemia. The representative blots were shown by cropping from different parts of the same bolt for HIF-1α and CD 34. The full-length blots are presented in Supplementary Fig. S5. The graphs show the fold-increase or -decease in each protein expression in the IR kidneys compared with that of the sham. Values are means ± SE. *P < 0.05 vs. AT1a+/+ mice on the same day, §P < 0.05 vs. AT1a+/+ mice at 14 days postischemia, †P < 0.05 vs. AT1a−/− mice at 14 days postischemia.
