Figure 1 | Scientific Reports

Figure 1

From: Lipopolysaccharide-induced neuroinflammation disrupts functional connectivity and community structure in primary cortical microtissues

Figure 1

Custom injection mold positions the microtissue for live inverted imaging without the need for physical manipulation of tissues. (A) The injection mold design consists of a hollow tube with patterned end cap. The 6 holes in the endcap will form the 600 µm diameter agarose pegs of the microwell. (B) A cross section image of the injection molding apparatus. The injection mold (gray) is placed in the well, suspended to the correct height by a 3D printed holder (turquoise), and hot liquid agarose (red) is pushed into the hollow tube of the injection mold with a sterile syringe. (C) Liquid agarose moves through holes in the endcap and into the well. (D) When the agarose (red) is cooled, the mold can be gently pulled upward and removed. (E) Removal of the injection mold reveals the microwell with 6 pegs to produce the “trampoline” tissue shape. (D) This process places the microtissue (tan) around 350 µm from the bottom of the glass plate, within the working distance of most objectives.

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