Figure 6

LPS disrupts community structure at 5–9 days after exposure. At 2 h after treatment, there was no significant difference (p = 0.3074) between PBS and LPS in modularity (A), including no difference in the intra-modular (p = 0.3056, B) or inter-modular (p = 0.056, C) correlation coefficients. Schematic representations of connectivity data display the stronger intra-modular connections in blue (module 1) and yellow (module 2) than inter-modular connection (green) for both PBS treated (p < 0.0001, D) and LPS treated (p < 0.0001, E) samples at 2 h, a. At 1–3 days after treatment, there was no significant difference (p = 0.0505) between PBS and LPS samples in modularity (F), however, there was a significant increase (p = 0.0070) in intra-modular correlation of PBS (G). Inter-modular correlations remained the same at this time point (p = 0.2690, H). Schematics representing of connectivity data shows PBS treated samples (I) with slightly increased in intra-modular connections (p = 0.0622) and unchanged inter-modular connections (p = 0.8413), while LPS treated samples (J) show no changes from 2 h (intra, p = 0.5200; inter, p = 0.8524). At 5–9 days the modularity of LPS samples were significantly lower than PBS samples (p < 0.0001, K). There was a significant increase in LPS intra-modular correlations (p = 0.0133, L) and a super significant increase in inter-modular correlations (p < 0.0001, M). Schematic representations of modular connectivity data show PBS treated samples (N) were unchanged from 1–3 days (p = 0.6338), and significantly high intra modular correlations compared to 2 h (p = 0.0082), while LPS treated samples (O) show a large increase in connectivity, which increases inter-modular connections (p < 0.0001) and intra-modular connections (p < 0.0001). Significance was determined with unpaired, two tailed t-tests with p < 0.05 (*p < .05, **p < 0.01, ***p < 0.001, ****p < 0.0001).