Figure 1 | Scientific Reports

Figure 1

From: Development of a sensitive, quantitative assay with broad subtype specificity for detection of total HIV-1 nucleic acids in plasma and PBMC

Figure 1

Revised qPCR and RTqPCR quantification assay synthetic standard and formats. Created with BioRender.com. (a) Synthetic DNA plasmid template containing the 433–633 HIV-1 LTR insert was linearized by ScaI and used for standard curve generation in the HIV-1 LDAs. RNA transcripts from the linearized DNA plasmid were made using the T7 promoter site and were used as HIV-1 RNA controls. (b) Protocol for Non-nested qPCR on crude DNA lysates. (c) Protocol for semi-nested RT-qPCR on RNA extracts.

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