Figure 3
From: Cholesterol as a modulator of cannabinoid receptor CB2 signaling
GEF of CB2 expressed in: (a) E. coli membranes and (b) CHO membranes (Millipore EMD). 2 μg of total protein per assay. Each point represents an average of four independent measurements (n = 4). Ligands were added at a concentration of 2 μM to ensure saturation of the receptor, and G protein was added as described in “Methods”. The dotted line indicates the rates of activation of G protein in the absence of a ligand. The rates of activation with CP-55,9040 are set to 100%, and rates of activation in the presence of SR-144,528 to 0%.
