Figure 5 | Scientific Reports

Figure 5

From: Minor allele of rs55763075 located in MTHFR is associated with the risk of cognitive impairment after anesthesia via modulating miR-34b

Figure 5

MiR-34b precursors suppressed the expression of MTHFR mRNA and protein in the primary PBMCs of patients carrying AA genotype of rs55763705 rather than in the primary PBMCs of patients carrying GG genotype of rs55763705 (*P value < 0.05 vs. NC group). (A) The expression of MTHFR mRNA was inhibited by miR-34b precursors and MTHFR siRNA in primary PBMCs of patients carrying AA genotype of rs55763705. (B) The expression of MTHFR protein was inhibited by miR-34b precursors and MTHFR siRNA in primary PBMCs of patients carrying AA genotype of rs55763705. (C) The expression of MTHFR mRNA was not affected by miR-34b precursors in primary PBMCs of patients carrying GG genotype of rs55763705. (D): The expression of MTHFR protein was not affected by miR-34b precursors in primary PBMCs of patients carrying GG genotype of rs55763705. (the original WB blots were included in Supplementary files)

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