Figure 4 | Scientific Reports

Figure 4

From: Daphnetin ameliorates acute lung injury in mice with severe acute pancreatitis by inhibiting the JAK2–STAT3 pathway

Figure 4

Daphnetin Pretreatment Inhibited the Apoptosis in Lung Tissues. Representative images of immunofluorescence detection of the apoptosis markers of TUNEL in the lung tissues. Nuclei were counterstained with DAPI. The results for the quantitation of mean fluorescence intensity (MFI) in the red channel are presented. The histograms were created using GraphPad 5 software(GraphPad Prism 5.01 http://www.xdowns.com/soft/xdowns2009.asp?softid=49668&downid=60&id=52443). The quantitative analysis of immunostaining was used by ImageJ software(Image J 2 system software https://imagej.net/Downloads). CON group, l-arginine vehicle + daphnetin vehicle; SAP group, l-arginine + daphnetin vehicle; DAP group, l-arginine vehicle + daphnetin; SAP + DAP group, l-arginine + daphnetin. Data are presented as the mean ± SD. n = 6/group. **P < 0.01 versus CON group. ##P < 0.01 versus SAP group, n.s.P > 0.05 vs. CON group. Scale bars: 50 μm.

Back to article page