Figure 5

Upregulation of NFIB in neuroblasts of the Emx1-Cre Cic knockout mice. (A–C) Representative images of NFIB immunostaining (grey) in the dentate gyrus. Cells with the highest NFIB expression are found in the neurogenic subgranular zone. Scale bars = 50 µm. (D–F) Representative images of NFIB staining at higher magnification showing increased NFIB immunoreactivity and more NFIB^hi cells in the knockout mice. Scale bars = 10 µm. (G) Quantification of fluorescent staining intensity of NFIB in DCX⁺ cells. More than 150 cells per animal were analysed. Each data point represents the average value from multiple cells per animal. (H) Quantification of the percentage of DCX⁺ neuroblasts co-express high levels of NFIB. N = 3‒6 animals per group. (I–K) In the knockout mice, DCX⁺ cells abnormally located to the molecular layer express high levels of NFIB (yellow arrowheads). Scale bars = 20 µm. Data are presented in box-and-whisker plots showing all data points, where centre lines represent medians, box limits represent interquartile ranges, and whiskers represent minimum to maximum data ranges. Statistical analyses were performed with one-way ANOVA with Tukey’s post hoc test. *P < 0.05; **P < 0.01.