Figure 5 | Scientific Reports

Figure 5

From: Cross-sectional imaging of intestinal barrier dysfunction by confocal laser endomicroscopy can identify patients with food allergy in vivo with high sensitivity

Figure 5

Quantitative Cryptometry of the colonic architecture by CLE. (A) Mean, minimal and maximal vessel diameter were measured using the vessel detection plug in of the IC-Viewer which enables automatic detection of the vessels directly from each endomicroscopic recording frame based on fluorescence intensity detection. Detection threshold was manually set at 10 μm for vessel detection. Dashed white line: colonic crypts, red dashed line: vasculature in the lamina propria. (B) Intercrypt distance (white arrows) was defined, as the distance between the geometrical centers of neighboring crypts, as defined by the crypt lumen. Distances between all crypts in one images were quantified. (C) Total crypt diameter was defined as the distance between epithelial cells constituting the crypts measured in 2 axes crossing the centers of the crypts (white arrows). Parameters in B and C were measured using the IC-Viewer software version 3.8.6 (Mauna Kea Technologies, Paris, France). Dashed white line: colonic crypts.

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