Figure 5

Comparison of human serum antibody binding to TKO and 5GKO ipLDEC lines. Human sera from 20 patients on the kidney transplant wait-list were incubated with TKO (GGTA1/CMAH/β4galNT2) or 5GKO (GGTA1/CMAH/ β4galNT2/SLA-I α chain/B2M) ipLDEC. Subsequently, fluorescent conjugated anti-human IgG or IgM was used to evaluate human IgG or IgM binding to the cells, with mean fluorescence intensity (MFI). Mann–Whitney test was used to analyze the differences between TKO and 5GKO ipLDEC lines. Human serum IgG exhibited significantly decreased binding to 5GKO ipLDEC compared to TKO ipLDEC (p < 0.0001). Human serum IgM binding to 5GKO ipLDEC was also significantly reduced compared to TKO ipLDEC, (p = 0.0443). Unfilled dots and triangles represented low PRA (< 10%) serum samples (n = 2). All other serum samples (n = 18) shown in dots were from individuals with high PRA (> 90%).