Table 1 Sequences of gRNA target and gRNA oligos.
Gene | gRNA target | gRNA oligo |
|---|---|---|
GGTA1 | GAGAAAATAATGAATGTCAA | 5′-CACCGAGAAAATAATGAATGTCAA-3′ 5′-AAACTTGACATTCATTATTTTCTC-3′ |
CMAH | GAGTAAGGTACGTGATCTGT | 5′-CACCGAGTAAGGTACGTGATCTGT-3′ 5′-AAACACAGATCACGTACCTTACTC-3′ |
β4galNT2 | CTGTATCGAGGAACACGCTT | 5′-CACCGTGTATCGAGGAACACGCTT-3′ 5′-AAACAAGCGTGTTCCTCGATACAC-3′ |
B2M | CTCGTGGCCTTGGTCCTGCT | 5′-CACCGTCGTGGCCTTGGTCCTGCT-3′ 5′-AAACAGCAGGACCAAGGCCACGAC-3′ |
SLA-1,2,3 (1) | ATCATGTACGGCTGCGACGTG | 5′-CACCGTCATGTACGGCTGCGACGT-3′ 5′-AAACACGTCGCAGCCGTACATGAC-3′ |
SLA-1,2,3 (2) | ACTATTGGGATGAGGAGACGCGG | 5′-CACCGCTATTGGGATGAGGAGACG-3′ 5′-AAACCGTCTCCTCATCCCAATAGC-3′ |
