Figure 4 | Scientific Reports

Figure 4

From: Serine residues 726 and 780 have nonredundant roles regulating STAT5a activity in luminal breast cancer

Figure 4

Effect of single-point phospho-deficient STAT5a mutants on characteristics of MCF7 cells. (A) Representative images and quantification of number and size of the colonies formed in soft agar by indicated MCF7 cells with knockdown (KD) of STAT5a rescued by phospho-deficient mutants. n = 3, *p < 0.02 compared to WT-STAT5a. (B) Proliferation in serum-free media (SFM) supplemented with PRL was assessed using XCELLigence, cell index indicates proliferation. n = 3, p < 0.02 compared to WT-STAT5a. (C) Immunoblots of T47D cells (top) and MCF7 (bottom) cells carrying transient rescues of STAT5a point mutants assessed for apoptosis by expression of cleaved caspase 3 or 7. Graphs and statistics generated in GraphPad Prism 9.0.0 for Windows (GraphPad Software, San Diego, California USA, www.graphpad.com).

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