Figure 3
ELISA studies of the sera in FIA-CIA (A) Antibody titers against the immunizing antigens bCII (brown), hFib (violet) and citrullinated fibrinogen (dark red) on day 84, at the end of the experiment. Repeated measurements were carried out for all antibodies, and showed rapid increases of anti-fibrinogen, anti-collagen and anti-citrullinated fibrinogen antibodies to high values after 14–35 days, which did not change thereafter. For reasons of clarity we only show the values obtained at the final blood sampling upon sacrifice. Antibody titers of native control animals are shown in black, and were negative (zero values) for all 3 investigated antibodies. (B) Antibodies against CCP, orange: immunized; blue: native control; *p < 0.05, **p < 0.01; error bars indicate SEM. Repeated measurements are shown for anti-CCP antibodies due to their variation over time.
