Figure 3 | Scientific Reports

Figure 3

From: Secretome screening reveals immunomodulating functions of IFNα-7, PAP and GDF-7 on regulatory T-cells

Figure 3

IFNα-4, IFNα-10 and IFNα-14 destabilise Treg markers whereas IFNα-7 stabilises CTLA4 expression. (A) Heatmap over mean effect on CTLA4 protein expression, (B) heatmap over mean effect on FOXP3 protein expression, (C) Heatmap over mean effect on cell viability, (D–E) Effect of IFNα-10 treatment on CTLA4 and FOXP3 expression, (F–G) Effect of IFNα-7 treatment on CTLA4 and FOXP3 expression. Tregs were from multiple donors (Donor 1 filled circle ●, Donor 2 empty circle ○, Donor 3 empty square □). The effect on FOXP3 and CTLA4 expression was normalized to % activity as compared to the MFI of live cells treated with the on-plate PBS neutral controls.

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