Figure 2

Motif analyses of the detected Kac sites. (A) Acetylation sequence motifs for amino acids around Kac sites (− 10 to + 10). The letter height represents the frequency of that amino acid residue at that position. The K in the middle position corresponds to the acetylated lysine. It was performed with motif-x (Version 1.2 10.05.06; https://motif-x.med.harvard.edu/motif-x.html). (B) Heat map analysis of the amino acid compositions around the acetylated sites. Red indicates an amino acid that is significantly enriched, while green indicates an amino acid that is significantly reduced. It was performed with the “heatmap.2” function in the “gplots” R-package. (C) Secondary structure analysis of Kac peptides. (D) Predicted surface accessibility of Kac peptides. Secondary structure and surface accessibility analysis were performed using NetSurfP (version 2.0; http://www.cbs.dtu.dk/services/NetSurfP/).