Figure 3

Pretreatment with a gradient concentration of WZYZ could reduce the apoptosis of germ cells induced by acute THS. Germ cell apoptosis was detected by TUNEL. The typical image shows that the brown area is TUNEL positive germ cells (black arrow). Bar = 100 μM. (a) Control group; (b) Model group; (c) Low-dose WZYZ group; (d) Middle-dose WZYZ group; (e) High-dose WZYZ group; (f) bar chart showing percentage of TUNEL positive area. The data showed an average ± SEM (n = 5 rat per group). **Compared with the control group, P < 0.01; compared with the model group, P < 0.05, P < 0.01.