Figure 3 | Scientific Reports

Figure 3

From: Lipidated PrRP31 metabolites are long acting dual GPR10 and NPFF2 receptor agonists with potent body weight lowering effect

Figure 3

GPR10 and NPFF2R profiling of in vivo metabolites of GUB03385. (a) IP1 measurement in human GPR10 Chem 1 cells (HTRF technology, CisBio) after 30 min. incubation at 37 °C with metabolites and the native peptide PrRP31. (b) cAMP measurements in human NPFF2R CHO-K cells (HTRF technology, CisBio) at a fixed concentration of 3 µM forskolin after 30 min. incubation at room temperature with metabolites and the native peptide NPSF. Representative curves of three determinations.

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