Figure 4

Monosialic acid, oligosialic acid, soluble polySia avDP20, and soluble long chain polySia was co-incubated with 10% NHS. Afterwards, activation of the alternative pathway was measured by detection of TCC formation using the ELISA. PolySia avDP20 reduced TCC formation in a dose-dependent manner. Data are shown as boxplot (min./Q1/median/Q3/max.) of n ≥ 3 independent experiments, and are normalized to normal human serum. One-way ANOVA followed by Bonferroni post hoc test; *p < 0.05, ***p < 0.001. NHS normal human serum.