Figure 3

Level of L. salmonis labial gland protein (LsLGP) transcripts during development and starvation related to the LsEF1α and LsADT3 reference genes (N = 5). (a–e) Developmental expression in fertilized egg sacs (egg), nauplius (nau) I and II, planktonic copepodids (c free) and copepodids 2 days (c 2d) and 4 days (c 4d) post infestation, chalimus (chal) I and II, pre-adult (pad) I and II and adult lice (ad). For female adult lice both young (Y) and mature (M) lice were analyzed. mRNA level was related to that of fertilized egg sacs. Statistically significant differences are denoted with different letters (p ≥ 0.05). (f–j) Expression in adult females at 0, 24 and 48 h (h) after removal from salmon. mRNA levels were related to that of the lice taken directly off the host (0 h).