Figure 7

Analysis of LsLGP3 and 4’s role in blood coagulation. (a) Coagulation efficiency in control (CTR), recLGP3 (75 μg) and synLGP4 (50 and 75 μg) treated plasma measured as time to plasma gel formation after thromboplastin addition (minutes ± SD; N = 3). Some synLGP4 treated samples did not coagulate during the experiment and was set to 45 min. (b–e) Knock down (KD) study of LsLGP4 during the mobile salmon louse stages to analyse the importance of LsLGP4 in blood feeding. (b) Relative transcript level (2^−∆∆Ct ± SD) of LsLGP4 in control (dsCTR) and dsLGP4 treated lice at 32 dpi, related to the LsEF1α and LsADT3 reference genes (N = 5). (c) Average number of recovered lice per fish (± SD) in fish (N = 3) infested with 10 control or dsLGP4 treated lice/fish. (d) Percentage of lice with visible blood in intestine at sampling in control and dsLGP4 treated lice (dsCTR N = 16, dsLGP4 N = 18). (e) The body length (BL) and egg string length (EL) of control and dsLGP4 treated lice (mm ± SD, dsCTR N = 16, dsLGP4 N = 18). Significant difference between control and treatment group are denoted with one asterisk (*) if p ≥ 0.05, two asterisk if p ≥ 0.01 and three asterisk if p ≥ 0.001.