Figure 5

Western blot analysis confirming the recognised EV markers according to the MISEV guidelines 2018⁵. Western blot analysis shows the protein content of EVs isolated from human plasma (n = 3 replicates) and feline plasma (n = 3 replicates). Molecular weight markers are also shown. EVs from both humans and felines concentrated, lysed, and subjected to western blot for recognised EV markers; Alix, Calnexin, HSP70, CD63 and APOA1. Representative images are shown.