Figure 7

Asymmetrical flow field flow fractionation (AF4) of human and feline EVs. Asymmetrical field flow fractionation (AF4) was carried out on human and feline EVs. (a) Shows 90° light scatter signal (full lines, left hand scale) overlayed on the radius of gyration (dotted lines, right hand scale). Red and black lines represent the human EVs showing two distinct populations of EVs. One in the small EV range (< 200 nm) and another population of larger particles (> 200 nm). In contrast, the feline EVs indicated by blue and green lines show peaks that indicate only a small EV population only (< 200 nm). Radius of gyration (Rg) determined from light scattering signals was recorded from human and feline EV samples. These size differences show that the density of human EVs is different to the density of feline EVs, suggesting that their structure and shape are different. (b) Shows the radius of gyration (dotted lines, right hand scale) overlaid on UV detector signal (full lines, left hand scale) which shows the early eluting protein populations of the samples. The protein pattern of the human EVs (shown in black and red) was similar to feline EVs (blue and green).