Figure 3 | Scientific Reports

Figure 3

From: Targeted therapy of pyrrolo[2,3-d]pyrimidine antifolates in a syngeneic mouse model of high grade serous ovarian cancer and the impact on the tumor microenvironment

Figure 3

Folate transporter expression and function in BR-5 and BR-Luc syngeneic HGSOC mouse models. Transcript levels for PCFT, RFC and FRα were measured by real-time RT-PCR for BR-5 (A) and BR-Luc (B) cells. Transcripts were normalized to β-actin transcripts and levels are shown relative to those in mouse liver (assigned a value of 1). (C) Total surface FRα was measured by titration with [3H]folic acid at 0 °C with and without unlabeled 10 µmol/L non-radioactive folic acid. (D) PCFT uptake was assayed using [3H]MTX (0.5 µM) at pH 5.5 at 37 °C in the absence and presence of 10 µM non-radiolabeled AGF94, as previously described22. Results are presented as mean values (± standard errors) from at least three experiments.

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