Figure 8

Immunofluorescence staining of FRβ-expressing tumor-associated macrophage population following treatment with AGF94. Slides were stained with FRβ (Genetex) with Alexa Fluor 488 secondary antibody and pan-Macrophage, F4/80, conjugated to APC-R700 (BD Biosciences). Images were acquired by confocal microscopy. Immunofluorescence staining is shown for representative tumor sections from control mice and mice treated with 4 doses of AGF94. The scale is 20 µm.