Figure 1 | Scientific Reports

Figure 1

From: Preparation of recombinant glycoprotein B (gB) of Chelonid herpesvirus 5 (ChHV5) for antibody production and its application for infection detection in sea turtles

Figure 1

Generation of recombinant partial gB proteins. Five antigenic fragments of gB protein, labeled gB-F1, gB-F2, gB-F3, gB-F1–2 and gB-F2–3, were expressed in E. coli under IPTG treatment (Lanes 1, 2, 3) and purified by Ni–NTA affinity chromatography. Purified proteins (Lane 3) with the expected molecular weight are indicated as asterisks. Five gB fragments were expressed as a fusion protein with a six-histine tag at its C-terminus, which was confirmed by western blot analysis with an anti-histidine antibody (shown in the panel below SDS-PAGE). Line—indicates the mock control lysate without IPTG induction, and Lanes 1 and 2 are the total and insoluble fractions of bacterial lysate, respectively. (For gB-F1, gB-F2 and gB-F3, not the whole blot was used to react with antibody. Due to the molecular weights of target proteins gB-F1, gB-F2 and gB-F3 are very small (around 10 kDa), the protein-transferred membrane was trimmed to the region between 15 and 10 kDa below for further antibody hybridization).

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