Figure 6
From: Monitoring longitudinal disease progression in a novel murine Kit tumor model using high-field MRI
Quantification of the splenic iron load. (a) Example of a control spleen tissue and (b) of a mutant spleen tissue stained with Prussian blue. (c) The training region within a male control originally used for manual annotation with clearly identifiable, positively stained areas. (d) An example of the achieved performance of the trained pixel classifier, iron-loaded areas highlighted in dark blue. (e) Results of quantitative image analysis of spleen samples (females: 4/4, males: 4/4; age: 12 month) stained with Prussian blue confirmed the unchanged splenic iron load in C3H KitN824K/WT mice and the general sex-specific iron status. Consistently, shorter T2 times were measured in the spleen of females compared with males, both at 12 months of age (**, p ≤ 0.01).
