Figure 5
From: Fat3 regulates neural progenitor cells by promoting Yap activity during spinal cord development
Overexpression of Yap recovers Fat3 loss-of-function effect. (A) In ovo electroporation of Flag-Yap together with sh-Fat3 was performed at HH13, followed by immunostaining analyses with indicated antibodies for chick embryos (thoracic level). +, electroporated side. GFP expression indicates transfected cells. Quantifications of Sox2+ and pH3+ cells in the electroporated side (+) relative to those in the unelectroporated control side (−) and the number of ectopic Sox2+ and pH3+ cells in the electroporated side were shown. Quantification was performed with 9–15 images from 4 to 6 embryos of each test set. Values are means ± sem. Statistical difference was determined by one-way ANOVA followed by Turkey’s test using Prism 9 (GraphPad) software; *p < 0.05, ****p < 0.0001. Scale bars: 100 μm. (B) Immunostaining analyses with anti-phospho Yap antibody showed reduced progenitor ___domain in the ventral ventricular zone in E11.5 Fat3f/f;N-Cre embryos compared to their littermate controls. Scale bars: 100 μm.
