Figure 2 | Scientific Reports

Figure 2

From: Amelioration of injury-induced tissue acidosis by a nonsteroidal analgesic attenuates antinociceptive effects of the pH-dependent opioid agonist NFEPP

Figure 2

Effects of subcutaneous (s.c.) diclofenac on nociceptive responses, inflammation and tissue pH. The paw pressure threshold (PPT) values after daily s.c. diclofenac (DCF; 20 mg/kg) injections in inflamed (A) and in contralateral non-inflamed (B) hindpaws, assessed at 4 days after intraplantar (i.pl.) complete Freund's adjuvant (CFA) inoculation before (0) and up to 240 min after the last DCF injection. *P < 0.05, **P < 0.01, ***P < 0.001 DCF versus vehicle, two-way RM ANOVA and Bonferroni tests. (C) Decrease of inflamed paw volume after daily s.c. DCF (20 mg/kg) injection measured for 4 days after i.pl. CFA inoculation after each DCF injection. *P < 0.05, **P < 0.01 DCF versus vehicle, two-way RM ANOVA and Bonferroni test. (D) pH values in inflamed paw tissue after DCF treatment (20 mg/kg s.c.) at 4 days after i.pl. CFA inoculation. **P < 0.01 DCF versus vehicle, two-tailed unpaired t-test; +++P < 0.001 inflamed versus non-inflamed paw, two-tailed paired t-test. Data show means ± SEM (n = 8 rats per group).

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