Figure 3
From: BMP2 promotes lung adenocarcinoma metastasis through BMP receptor 2-mediated SMAD1/5 activation
Inhibition of BMP2 reduces invasiveness and downregulates EMT in lung cancer cells. (A) The invasion and migration abilities were reduced in BMP2-depleted CL1-5 and AS2 cells. BMP2 expression was depleted by two specific shRNAs. shLacZ is the non-targeting control. Slides were observed under 10 × magnification. (B) Quantitative data derived from (A). Bar graphs represent the average number of migrated cells ± SD. At least three fields were counted and averaged for each cell line. (C) The protein levels of BMP2 and EMT marker were determined by western blotting in CL1-5 and AS2 cells. (D) Cell invasion and migration abilities were reduced in BMP2-depleted CL1-5 and AS2 cells. The expression of BMP2 was depleted by siRNA. A non-targeting siRNA was used as a control. Slides were observed under 10 × magnification. (E) Quantitative data derived from (D). Bar graphs represent the average number of migrated cells ± SD. At least three fields were measured in each cell line. (F) The expression levels of BMP2 and EMT markers were determined by western blotting. The p-value was determined by Student’s t test. (*p < 0.05, **p < 0.01, ***p < 0.001).
