Figure 22 | Scientific Reports

Figure 22

From: The autism risk factor CHD8 is a chromatin activator in human neurons and functionally dependent on the ERK-MAPK pathway effector ELK1

Figure 22

CHD8 co-immunoprecipitates with ELK1 in human neurons, and its chromatin binding perturbed in the absence of ELK1. (a) Pull-down assay for CHD8 and western blotting of CHD8 and ELK1 in human neurons (also see two other experimental replicates in Fig. 23a). (b) Knockdown of ELF4 does not affect CHD8 binding on either of ETS or YY1 motif sites, suggesting ELF4 does not influence chromatin binding of CHD8. (c) ChIP-qPCR for CHD8 binding after ELK1 knockdown with two different hairpin RNAs (shRNAs). Control condition is an empty vector. The number of ETS motif at each peak region indicated beneath each peak. There was no change in CHD8 binding at sites without the ETS motif. See also Fig. 25c for validation of CHD8 peaks in CHD8-KO neurons.

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