Figure 2 | Scientific Reports

Figure 2

From: Expression of LIM ___domain-binding 3 (LDB3), a striated muscle Z-band alternatively spliced PDZ-motif protein in the nervous system

Figure 2

Immunolocalization of LDB3 in different regions of mouse brain. (A) Representative immunofluorescence using GTX115593 antibody on motor cortex coronal section shows LDB3 signal (green) in the apical dendrite bundles probably from layers III and V stained by MAP2 (red) and in thinner dendrites not labeled by MAP2 which are likely from superficial neurons in six-month old mice (n = 6). Bottom panel shows LDB3 signal (green) in MAP2 (red) stained cell body and dendrites of pyramidal neurons in motor cortex layer V in the same coronal section. (B) Representative immunofluroscence shows the same LDB3 antibody signal (green) co-localized with α-actinin-2 signal (red) at the Z-discs in the vastus lateralis muscle fibers of 18 day old Ldb3+/+ embryo but not Ldb3−/− littermates (n = 3). Note Ldb3−/− mice do not survive beyond birth. (C, D) Representative immunofluorescence using the same antibody shows LDB3 (green) in MAP2 (red) stained dendrites from the hippocampal CA1 neurons (C) and in calbindin (red) stained dendrites from the cerebellar Purkinje cells (D) in 6 month old mice (n = 6). LDB3 signal is absent in parallel sections incubated with LDB3 antibody plus a blocking peptide. Scale bar in all images 50 µm.

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