Figure 3

The effect of betulinaldehyde on the SQSTM1 and LC3-II levels of A549 cells. (A) A549 cells were treated with different concentrations of betulinaldehyde for 24 h before the cell extracts were analyzed using the western blotting technique. The statistical analyses of the (B) SQSTM1 levels and (C) LC3-II levels are included. Cropped images are displayed, uncropped blots are displayed in Supplementary Fig. 2. The images were collected from different parts of the same gel with same loading amounts. The blot gels shown are representatives of three different experiments (n = 3). All values are expressed as the mean ± SD (n = 3). Statistical significance was evaluated by one-way analysis of variance followed by LSD for post hoc test. *P < 0.05, **P < 0.01 versus 0 μM group.