Figure 5

Distribution histogram of reported physiological NAD⁺ levels in normal mouse and rat liver. Mean NAD⁺ values measured using different quantification methods in (a) mice and (b) rat liver sorted from lowest to highest value (nmol/g of tissue weight). The represented data was collected from young control mice (< 14 months old) and rats (< 18 months old). For each study that included more than one measurement per tissue, the datapoints are labeled using a number that represent the study, respectively: 1: Gaikwad et al. (2001), 2: Dall et al. (2019), 3: Trammell et al. (2016), 4: Dietrich et al. (1968), 5: Ballard (1971), 6: Slater, Sawyer & Sträuli (1964), 7: Wendt et al. (2019), 8: Kiehlbauch et al. (1993). Arrows indicate LC–MS measurements that included the use of internal controls. NAD⁺ levels in (c) mouse and (d) rat liver segregated by quantification method. A One-way ANOVA with Sidak post hoc test was conducted to compare effect of the quantification methods on NAD⁺ levels and did not show any significant differences between methods.