Figure 5
From: Ischemic stroke protected by ISO-1 inhibition of apoptosis via mitochondrial pathway
TUNEL staining analysis of apoptotic cells as well as the detection of apoptotic proteins. (a) Apoptotic cells were labeled with fluorescein isothiocyanate (red), and all nuclei were stained with DAPI (blue). Bar 50 μm. (b) Proportion of apoptotic cells in total cells. (c–f) Expression levels of Bax, caspase3, and Bcl2 protein were determined by Western blotting. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
