Figure 1
From: Axl alleviates DSS-induced colitis by preventing dysbiosis of gut microbiota
The expression of Axl was upregulated in DSS-induced colitis. (a) Changes in body weights were measured during the administration with 2.5% DSS for 8 days. Data are represented as a percentage of body weight relative to initial body weight. (b) DAI scores were evaluated based on body weight loss, stool consistency, and bleeding in the stool. (c) The macroscopic appearance of the colon (left panel) and the colon length (right panel) were measured on day 8 after DSS administration. (d) Representative microscopic images (magnification 200 ×) of colon tissues stained with H&E (left panel). The histological scores were evaluated according to the degrees of epithelial damage, edema, hyperplasia, and immune cell infiltration (right panel). (e) mRNA expression of Axl and Gas6 was analyzed by qRT-PCR in the small intestine, colon, and MLN. (f) The expression of Axl and Gas6 was evaluated by immunohistochemistry in the colon tissue. The brown-colored areas indicate the expression of Axl or Gas6. Data are shown as the mean ± SEM values. Significance (*p < 0.05, **p < 0.01, ***p < 0.001) was compared with control mice, calculated by the Student's T-test (a, c, e) or Mann–Whitney test (b, d). Data are representatives of three independent experiments (n = 3 mice per group).
