Figure 5
From: Axl alleviates DSS-induced colitis by preventing dysbiosis of gut microbiota
A deficiency of Axl exacerbated DSS-induced colitis by inducing an abnormal expansion of Proteobacteria. (a) Body weights and (b) DAI scores were measured every day during 2.5% of DSS administration for 5 days. (c) The macroscopic appearance of the colon (left panel) and the colon length (right panel) was measured on day 5 after DSS administration. (d) Representative microscopic images (magnification 200 ×) of colon tissues stained with H&E (left panel) and the histological scores (right panel) were evaluated as described in “Materials and methods”. (e) The populations of IFNγ-secreting activated Th1 cells were determined in the colon using flow cytometry analysis (left panel), and the absolute number of IFNγ-secreting activated Th1 cells was represented as a bar graph (right panel). (f) The bacterial load and (g) the relative number of bacteria were determined as described in “Materials and methods”. Significance (*p < 0.05, **p < 0.01, ***p < 0.001) was compared with each group and calculated by the one-way ANOVA. Data are representatives of three independent experiments (n = 3 mice per group).
