Figure 12

CircDYRK1A inhibited the proliferation, invasion, and migration of GC cells in vitro. (A) Over-expression of circDYRK1A powerfully prevented the proliferative capacity of GC cell line MGC-803. The X-axis represents the time (h) after transfection at five time points of 0, 24, 48, 72, and 96 h, respectively. The Y-axis represents the optical density (OD) value per well at a wavelength of 450 nm (OD 450). In the MGC-803 cell line, compared with the corresponding control (P < 0.0001) and NC (P < 0.001) groups, the absorbance values of the OE group were significantly lower. (B) Over-expression of circDYRK1A in infected MGC-803 suppressed the capability of GC cells to invade. Under the 200 × view of light microscope field, the OE group had fewer cells invaded the lower chamber of the Transwell than the corresponding control and NC groups. (C) Over-expression of circDYRK1A in infected MGC-803 checked the number of invaded cells. By counting the cells, the average number of the control group was 104.40 ± 7.60, the average number of the NC group was 95.40 ± 5.41, and the average number of the OE group was 54.00 ± 5.48. (D) Over-expression of circDYRK1A in infected MGC-803 suppressed the capability of GC cells to migrate. Under the view of inverted microscope, the healing speed of scratches in the OE group was significantly lower than that in the corresponding control and NC groups at 0 and 48 h. The scale was 100um. (E) Over-expression of circDYRK1A in infected MGC-803 checked the rate of migrated cells. The Y-axis represents the rate of migrated cells. By calculating the mobility, the control group is 60.64 ± 1.66, the NC group is 57.20 ± 12.41, and the OE group is 30.26 ± 2.29 (P < 0.01).