Table 2 Sensitivity, specificity, positive and negative predictive values and likelihood ratios for the detection of any bacteria on mNGS compared to organism isolation by culture.
Test methods | Sensitivity* (%) | Specificity* (%) | PPV* (%) | NPV * (%) | PLR* | NLR* | jω |
|---|---|---|---|---|---|---|---|
Culture of DT + SF | 58.6 (45.0–71.1) | 94.6 (80.5–99.1) | 94.4 (80.0–99.0) | 59.3 (45.8–71.7) | 10.8 (2.8–42.5) | 0.4 (0.3–0.6) | 0.46 |
Culture of DT | 58.6 (45.0–71.1) | 100 (88.3–100) | 100 (87.4–100) | 60.7 (47.3–72.7) | Infinity | 0.4 (0.3–0.6) | 0.50 |
Culture of SF | 48.3 (35.1–61.7) | 94.6 (80.5–99.1) | 93.3 (76.5–98.8) | 53.8 (41.1–66.1) | 8.9 (2.3–35.3) | 0.5 (0.4–0.7) | 0.34 |
mNGS of DT + SF | 94.8 (84.7–98.7) | 89.2 (73.6–96.5) | 93.2 (82.7–97.8) | 91.7 (76.4–97.8) | 8.8 (3.5–22.2) | 0.1 (0.02–0.2) | 0.85 |
mNGS of DT | 93.1 (82.5–97.8) | 97.3 (84.2–99.9) | 98.2 (89.0–99.9) | 90.0 (75.4–96.7) | 34.4 (5.0–238.4) | 0.1 (0.02–0.2) | 0.90 |
mNGS of SF | 86.2 (74.1–93.4) | 89.2 (73.6–96.5) | 92.6 (81.3–97.6) | 80.5 (64.6–90.6) | 8.0 (3.1–20.2) | 0.2 (0.1–0.3) | 0.75 |
