Figure 1
From: YB1 modulates the DNA damage response in medulloblastoma
YB1 is expressed across all MB subgroups and overexpression is associated with shortened survival in an SHH primary mouse model (A) Immunoblotting cell lysate from SHH cells (MBC (primary NestinD2-SmoA1), Pzp53Med (Ptch-LacZ-p53null), Daoy, UW228, and ONS-76), and group 3/4 cells (D341, D556, BT52, D283, and CHLA01) with GAPDH as control. (B) Immunohistochemistry of SHH subgroup samples from both TP53 wild type and mutated patients showing positive staining of both Nestin (Stem marker) and YB1 (Left scale bar = 100 μm, quantification Supp Fig. 1a and b). (C) Survival analysis of BL6 mice orthotopically implanted with NestinD2-SmoA1 primary cells following adenoviral overexpression of YB1 (GFP median survival 26.5 days YB1 median survival 60.5 days p < 0.0001). (D) UMAP of previously published single cell sequencing analysis of SHH-Math-Cre-SmoM2 showing enrichment of YB1 in numerous cell populations collected from UCSC Cell Browser: active cell cycling (MS-A1 and MS-A2) and progenitor (MS-B1). Expression profile is subdivided into 10 expression ranges apart from no expression and percent of all cells within each range listed on right.
