Figure 6
From: YB1 modulates the DNA damage response in medulloblastoma
YB1 depletion results in greater canonical NHEJ and lower HR (A) Schematic of distal EJ without indels assay whereby two sgRNAs are co-transfected with SCEI to generate blunt ends repairable through cNHEJ to restore GFP expression. (B) Western blot of YB1 KD in EJ7-HEK cells. (C) EJ7 cNHEJ assay (shSCR vs shYB1, 95% CI = −0.46–(−0.07) p = 0.0119, shSCR vs shTP53BP1 95% CI = 0.046–0.43 p = 0.0197, one-way ANOVA, n = 3). (D) Western blot of YB1 KD in U2OS DR-GFP cells. (E) U2OS DR-GFP assay (shSCR vs shYB1 95% CI = 0.1975–0.6395 p = 0.0006, shSCR vs shCtIP 95% CI = 0.1858–0.6278 p = 0.0007, one-way ANOVA, n = 2).
