Figure 6

KYNU and ADAR1 interaction in aggressive breast cancer cell and subcellular localization. (A) MDA-MB-231 cell were lysed and immunoprecipitations were performed using a rabbit polyclonal antibody against ADAR1 or control normal rabbit IGg. Western blotting was carried out using a rabbit polyclonal antibody against KYNU (upper panel). Membrane was reprobed using a rabbit polyclonal against ADAR1 (lower panel); (B) Confocal immunofluorescence images of KYUN (red), ADAR1 (green) (using mouse monoclonal antibody against ADAR1) and nucleus (Dapi) (blue) of breast cancer cell MDA-MB-231. Scale bars, 150 μm.