Figure 3 | Scientific Reports

Figure 3

From: Identification of protein phosphatase 4 catalytic subunit as a Wnt promoting factor in pan-cancer and Xenopus early embryogenesis

Figure 3

PPP4C correlates with canonical Wnt signaling both in tumors and X. laevis embryos. Enrichment of genes in the Wnt upregulated (a) and downregulated (b) gene sets by GSEA in LGG. (c) Schematic diagram of axis duplication assay. Embryos were microinjected with indicated reagents at stage 3 and cultured until tailbud stage. (d) Schematic diagram of Wnt target gene induction. Embryos were injected at stage 1, AC were dissected at stage 8 and cultured till the sibling embryos reached stage 10.5. n = 30. (e) Representative phenotype of (c). Embryo images in (c,d) were downloaded from Xenbase website48. (f,h) Quantitative results of axis duplication by indicated reagents ns, p ≥ 0.05; *p < 0.05; **p < 0.01. Student t-test. (g,i) RT-PCR analysis of animal cap tissue microinjected with indicated reagents. odc1 was loading control. WE whole embryo, -RT without reverse transcriptase, Con. uninjected control. Reagents used in (f–i) WT CTNNB1 mRNA (WT-BC), 50 pg; CA CTNNB1 mRNA (CA-BC), 20 pg; Co MO, 30 ng; ppp4c MO, 30 ng; Re.ppp4c mRNA, 250 pg.

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